The FillHoles option will not fill holes larger than the maximum cell diameter, squared.FillHoles now fills holes that would have not otherwise been filled, for instance if you have a confluent field of cells, all holes in the thresholded image would be filled by the corrected version. IdentifyPrimaryObjects – (Issue # 1133) IdentifyPrimaryObjects fills holes twice – once after thresholding and once after separating touching objects.IdentifyPrimaryObjects / IdentifySecondaryObjects – Do not smooth the image when thresholding by a measurement or using manual-mode.The former calculation is preserved as “Log Transform (legacy)” and all old pipelines use that instead of the new log-transform calculation. ImageMath: The log-transform option now performs log2(x+1) which transforms the range, 0-1, to the range 0-1.The ClassifyPixels module is loaded on platforms that do not support it, but it displays an error status.Similarly File / Export / Pipeline appends “.cppipe” (issue #918) On OS/X, “.cpproj” is appended to the file name entered in File / Save Project As unless the filename already ends in “.cpproj”.Use “-p” for both pipelines and projects – CellProfiler will figure out what to do based on file type. The default choice for file output location for ExportToSpreadsheet and UntangleWorms has been changed to the default output folder to match the behavior of ExportToDatabase, SaveImages, CreateBatchFiles and MeasureNeurons (issue #1070).Better error messages if matching by order and NamesAndTypes finds more images in one channel than another (Issue #714).csv file has more fields than there are headers, ignore the extra fields instead of preventing the user from using the file (issue #853) csv file along with a setting that lets you disable the warning and silently overwrite the. Added a warning dialog that is displayed if your pipeline tries to overwrite a.Issue #1004 – added a setting that asks you if you want to use an HTTP prepend (previously, you’d leave the text blank to indicate that).Issue #1003 – warn user if SQLite is used with the CreateBatchFiles module (you can’t update a SQLite database that’s open in another process).Issue #1105 – added a keyboard shortcut to toggle between showing objects as outlines or using an alpha-blend of the image and colored object.Issue #1054 – added a button that toggles outlines off and on.Added setting that allows user to create an output image of just the numeric data on a black background (issue #978).Added a preference to optionally save the pipeline and / or file list when saving a project (Issue #1160).Show the group number and index of the image set within the group in the title of module display windows for pipelines that use grouping.The “-o” command-line switch will create the indicated output folder if it doesn’t exist (Issue 1025).If a module is marked as “paused” for test mode and the project or pipeline is saved and reloaded, the module remains marked as “paused” on loading.Use the color-picker setting to pick colors in OverlayOutlines, DisplayDataOnImage and ExportToDatabase.Added an “-omero-credentials” command-line switch to let CellProfiler authenticate with Omero in headless mode (issue #1052).Added ctrl-p shortcut to save the current pipeline to a file.
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